De novo Design

De novo design is an attractive approach for constructing designed proteins with predetermined structures and functions. Creative Biostructure provides de novo design related services for protein engineering, including protein structure prediction, protein folding and interactions, thermodynamics information, energetic state, stability, de novo meaning in biology etc.

De novo design requires deep understanding of the structure of a protein and the correlated functions. It is accomplished either from scratch or by the modular approach.

Linear chains of amino acids are adopted a unique 3D structure in the native surroundings to perform proteins. In de novo design, to identify amino acid sequences folding into proteins with desired functions is the ultimate objective. There are various approaches predicting protein structure including comparative modeling and fold recognition. In proteins, sequence similarity implies structural similarity. Accordingly, the method of comparative modeling is formed. The structure of a protein can be predicted by comparing the amino acid sequence to that of native 3D structure known. When the target and template share over 50% of sequence, the predictions are of high quality. Sequence similarity implies structural similarity but similar structures can be found for proteins with different sequence.