the design and synthesis of circular rnas

We use FBXW7 as an example. Both linear mRNA of FBXW7 and circ-FBXW7 can be transcribed in vivo. The convergent primer pair (blue) and divergent primer pair (red) are designed for PCR after the extraction of total RNA or genomic DNA. The PCR products are analyzed by electrophoresis. Both primer pairs can amplify the corresponding bands when using the cDNA produced by reverse transcription. If the total RNA is treated with RNase R, the convergent primer pair (blue) fails to form the bands near 100 bp. When the genomic DNA is used as the template, the divergent primer pair (red) can’t amplify the bands above 100 bp. When sequencing the amplifications in the red box, the circRNAs can be verified.

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